Composite

Part:BBa_K1792000:Design

Designed by: Brian Dempsey   Group: iGEM15_Lethbridge_HS   (2015-09-17)


Petal Death Protein (PDP) - Oxalic acid production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PDP gene is under control of the constitutively active medium transcription promoter J23107. It is possible to turn Oxalic acid production up or down by mutating this promoter to a stronger or weaker promoter.


Source

Petal Death Protein (PDP) from Dianthus caryophyllus (Carnation) (Clove pink). Based on the genomic sequence submitted to UniprotKB - Q05957 (PDP_DIACA).

References